T R A C K       P A P E R
ISSN:2394-3661 | Crossref DOI | SJIF: 5.138 | PIF: 3.854

International Journal of Engineering and Applied Sciences

(An ISO 9001:2008 Certified Online and Print Journal)

Effect of Macroporous Support Particles on Cell Immobilization, Mass Transfer and Rheology in a Stirred Cultivation of Aspergillus oryzae Using a Swingstir® Mixer

( Volume 5 Issue 10,October 2018 ) OPEN ACCESS
Author(s):

Narges Ghobadi, Chiaki Ogino, Naoto Ohmura

Abstract:

Immobilization of filamentous fungi via bioprocessing is a popular way to avoid mechanical damage to cells while increasing enzymatic activity. Passive immobilization is an efficient because chemical additives are not used during the immobilization process. In this study, passive immobilization of Aspergillus oryzae was performed in a stirred tank via a flexible agitator to improve the oxygen mass transfer and rheology while increasing the enzymatic activity. To elucidate the effect of porous biomass support particles on these factors, two particles with different sizes and populations were tested in separate batch cultivations. Results showed that one of the advantages of fungal immobilization is the prevention of a high rate of glucose consumption during a submerged fermentation process. Decreasing the size of the biomass support particle increased the shear-thinning behaviour of fluid. We also determined the optimal number of particles needed to produce the largest final amounts of immobilized biomass and enzyme activity.

DOI DOI :

https://dx.doi.org/10.31873/IJEAS.5.10.19

Paper Statistics:

Total View : 581 | Downloads : 572 | Page No: 56-62 |

Cite this Article:
Click here to get all Styles of Citation using DOI of the article.