The Minimum Inhibitory Concentrations exerted by the N-salicylidene-2-substituted aniline against various strains of phytopathogenic

Mohamed Yazid Belghit, Zohir Nedjar, Abdelhamid Moussi, Djamel Barkat

Fusarium genus includes several plant pathogenic fungi that may attack a large number of plants, causing diseases called fusarioses. In our strategy of the fight against this kind of fungus in vitro, we tested the acidity and the basicity of the culture medium (PDA) on mycelial growth of this genus of fungus, followed by the determination of the minimal inhibitory concentration of synthetic compounds (Schiff bases) such as N-salicylidene-2-methoxyaniline and N-salicylidene-2-nitroaniline against three species plant pathogenic: Fusarium culmorum, Fusarium graminearum and Fusarium verticillioides, using the agar dilution method at different concentrations of these two Schiff base derivatives and Tachigaren 30% SL as a standard fungicide. We found that the low acidity of the culture medium revealed better mycelial growth. The results show that the inhibition of mycelial growth seems to be proportional to the concentration of these compounds, however a better activity was revealed for the compound N-salicylidene-2-methoxyaniline with Minimum Inhibitory Concentration (MIC) =10 mg / ml against of the F. culmorum and F. graminearum strains. The two compounds tested showed a diversity of activity for all the fungal strains. This may be due to the presence of the various substituted groups (methoxy and nitro) on the structure of these compounds.