The Study of Antimicrobial, Anthelmintic and Cytotoxic activities of Parthenium Hysterophorus L.

Nitin Sharma, Mahesh Kumar, Usha Tiwari, Manoj Kalakoti

The present study has been designed to evaluate antimicrobial, anthelmintic and cytotoxic activities of Parthenium hysterophorus L. plant leaves extract. Present work reports for the antimicrobial activities of P. hysterophorus leaf. Dried samples were sequentially extracted with many solvents. Methanol, acetone, chloroform, petroleum ether and water extracts of leaf showed considerable antibacterial activity against Escherichia coli, Klebsiella pneumonia and also antifungal activity against Candida albicans. The in-vitro cytotoxic activity of methanolic extract was performed by MTT assay method against HeLa cell line. The methanolic, acetone, chloroform, petroleum ether, water extract (100 mg/ml concentration) of Parthenium hysterophorus plant leaves was taken for anthelmintic activity against Pheretima posthuma. Effect of inhibition of cell growth showed significant cytotoxicity against leaf methanolic extracts showed considerable inhibition (80–90%) of HeLa cell lines. The results obtained from the study indicate good anthelmintic activity against Pheretima posthuma. The present study concluded that the methanolic extract of Parthenium hysterophorus possess potent antimicrobial, anticancer and also anthelmintic activities.