Inhibitory Property of Crude Ethanolic Extract of Harungana Madagascariensis Root on New Castle

Mathew B.A, Yusuf M.N, Abu S.J, Fakolade B.A, Ibrahim A. A

It has been observed from the research work that Newcastle disease Virus has no cure and the prophylactic vaccines available are not potent enough to eliminate the Newcastle disease virus, thus, it is pertinent to carry out research on Newcastle disease virus in order to proffer solution to the menace. The aim of this study is designed to; determine the inhibitory property of ethanolic extract of Harungana madagascariensis  root on Newcastle disease virus in chicks, determine the histopathology of the extract on the chicks and to equally determine the number of organic components of the extract. The inhibitory property of the crude ethanolic extract Harungana madagascarieniss roots on Newcastle disease virus was studied in two weeks old chicks. 0.2ml of LD50 concentration of the virus was injected into the chicks intramuscularly. An hour later, after the infection of the Newcastle disease virus, they were fed with graded concentration of the extract (10mg/ml, 50mg/ml, 100mg/ml and 250mg/ml) and were observed for inhibitory/antiviral activity for 24-96 hours. Significant inhibitory property was observed (P <0.05) at the concentration of 100mg/ml of the extract. Therefore, the result shows that crude ethanolic extract of Harungana madagascariensis has inhibitory property at the concentration of 100mg/ml and the extract did not have any toxicological effect. Further studies should be done on the inhibitory property of the plant at other concentrations and purification of the plant for its chemical constituents should equally by studied.